Validation by a mass spectrometric reference method of use of boronate affinity chromatography to measure glycohemoglobin in the presence of hemoglobin S and C traits.
نویسندگان
چکیده
of Use of Boronate Affinity Chromatography to Measure Glycohemoglobin in the Presence of Hemoglobin S and C Traits, Randie R. Little, Hubert Vesper, Curt L. Rohlfing, Maria Ospina, Sekineh Safar-Pour, and William L. Roberts ( University of Missouri-Columbia School of Medicine, Department of Pathology & Anatomical Sciences, Columbia, MO; 2 Centers for Disease Control and Prevention, Atlanta, GA; 3 University of Utah, Department of Pathology, Salt Lake City, UT; * address correspondence to this author at: Department of Pathology & Anatomical Sciences, University of Missouri-Columbia School of Medicine, 1 Hospital Dr., Columbia, MO 65212; fax 573-884-8823, e-mail [email protected])
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A boronate affinity column method for the measurement of glycosylated hemoglobins was evaluated. In the procedure the glycosylated hemoglobins were bound by immobilized boronic acid to separate them from nonglycosylated hemoglobins. Elution of bound glycosylated hemoglobins was carried out with sorbitol buffer, and the absorbance was read at 414 nm. The method was linear to a glycosylated hemog...
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عنوان ژورنال:
- Clinical chemistry
دوره 51 1 شماره
صفحات -
تاریخ انتشار 2005